Caution
Concentrations of HEPES over 40mM may be toxic to some cell types.
Preparation Note
Prepared from HEPES free acid and cell culture grade water.
Application
HEPES is an organic zwitterionic buffering agent effective in
the physiological pH range of 6.8 to 8.2 (pKa 7.55). It is typically
used in cell culture at concentration between 5mM to 30 mM. This product
has been qualified for use in cell culture.